大鼠肝脏中3,3',5'-T3（反式三碘甲状腺原氨酸）脱碘生成3,3'-T2（二碘甲状腺原氨酸）的研究。

Studies on the deiodination of 3,3',5'-T3 (reverse T3) to 3,3'-T2 (diiodothyronine) in rat liver.

作者信息

Hüfner M, Grussendorf M

出版信息

Acta Biol Med Ger. 1980;39(2-3):169-75.

Abstract

Properties of the deiodination reaction of rT3 to 3,3' T2 in rat liver homogenate are reported and compared with T4 to T3 conversion under similar conditions. pH optimum and SH-group dependency of these two reactions are quite different, though both are concerned with 5' deiodination. The most potent activator of the reaction rT3 to T2' is dithiothreitol; the enzyme activity increases almost linearly even at very high concentration of this compound (the same is true for mercaptoethanol). Glutathione and coenzyme A, show only small activating effects. T4 to T3 and rT3 to T2' converting is being induced almost parallel in thyrectomized rats substituted with T4 or T3.

摘要

报道了大鼠肝脏匀浆中反式三碘甲状腺原氨酸（rT3）向3,3',5'-三碘甲状腺原氨酸（3,3'T2）脱碘反应的特性，并与相似条件下甲状腺素（T4）向三碘甲状腺原氨酸（T3）的转化进行了比较。尽管这两个反应都涉及5'-脱碘，但它们的最适pH值和对巯基的依赖性却有很大差异。rT3向T2'反应的最有效激活剂是二硫苏糖醇；即使在该化合物浓度非常高时，酶活性也几乎呈线性增加（巯基乙醇也是如此）。谷胱甘肽和辅酶A仅表现出较小的激活作用。在给予T4或T3替代的甲状腺切除大鼠中，T4向T3和rT3向T2'的转化几乎是平行诱导的。