Avioli R, Miller R A, Birge S J
Adv Exp Med Biol. 1980;128:399-404. doi: 10.1007/978-1-4615-9167-2_45.
A preparation of isolated intestinal cells has been developed which permits the incubation of these cells in culture for 90 min. with preservation of viability and responsiveness to vitamin D analogues added in vitro. Characterization of phosphate transport suggested the presence of two transport processes which differed in their affinity for phosphate, calcium stimulation, and pH dependence. Both processes were facilitated by sodium transport. This cell preparation promises to be a valuable experimental model for the elucidation of the action of vitamin D on phosphate transport.
已开发出一种分离肠道细胞的制剂，可使这些细胞在培养中孵育90分钟，同时保持活力以及对体外添加的维生素D类似物的反应性。对磷酸盐转运的特性分析表明存在两种转运过程，它们在对磷酸盐的亲和力、钙刺激和pH依赖性方面存在差异。这两种过程都由钠转运促进。这种细胞制剂有望成为阐明维生素D对磷酸盐转运作用的有价值的实验模型。
