Rahmatullah M, Boyde T R
Clin Chim Acta. 1980 Oct 23;107(1-2):3-9. doi: 10.1016/0009-8981(80)90407-6.
A rapid and reproducible method is described for measurement of urea in biological materials (after deproteinisation) and in serum (without deproteinisation). Urea is colorimetrically determined with diacetyl monoxime and thiosemicarbazide in the presence of sulphuric acid, phosphoric acid and ferric chloride. The sensitivity of the colorimetric reaction and stability of the colour are enhanced over existing related procedures and the serum blank diminished, enabling urea to be precisely measured in micro amounts (1--5 microliters) of serum.
本文描述了一种快速且可重复的方法，用于测量生物材料（脱蛋白后）和血清（未脱蛋白）中的尿素。在硫酸、磷酸和氯化铁存在的情况下，使用二乙酰一肟和硫代氨基脲通过比色法测定尿素。与现有相关方法相比，该比色反应的灵敏度和颜色稳定性得到了提高，血清空白值降低，从而能够精确测量微量（1 - 5微升）血清中的尿素。
