体外灌注的大鼠、兔、豚鼠、狗和仓鼠睾丸的睾酮分泌：与莱迪希细胞超微结构的相关性。

Testosterone secretion by rat, rabbit, guinea pig, dog, and hamster testes perfused in vitro: correlation with Leydig cell ultrastructure.

作者信息

Zirkin B R, Ewing L L, Kromann N, Cochran R C

出版信息

Endocrinology. 1980 Dec;107(6):1867-74. doi: 10.1210/endo-107-6-1867.

Abstract

Stereological methods were employed to determine volume and surface densities of cytoplasmic organelles in Leydig cells of hamster, rat, rabbit, dog, and guinea pig testes. Contralateral testes were perfused in vitro with maximally stimulating gonadotropin concentrations to determine the capacity of these testes to secrete testosterone. Significantly different amounts of testosterone were secreted by in vitro perfused testes of the five species when maximally stimulated with ovine LH. Significant differences also were seen in the volume and surface densities of smooth endoplasmic reticulum, mitochondria, rough endoplasmic reticulum, and lipids in Leydig cell cytoplasm of the five species. Most interestingly, linear positive correlations were seen between testosterone secretion and smooth endoplasmic reticulum volume (r = 0.99) and surface (r = 0.99) densities. Thus, virtually all of the differences in testosterone secretion by maximally stimulated testes of five species could be accounted for by between-species differences in the amount of Leydig cell smooth endoplasmic reticulum.

摘要

采用体视学方法测定仓鼠、大鼠、兔、狗和豚鼠睾丸间质细胞中细胞质细胞器的体积和表面密度。对侧睾丸在体外灌注最大刺激浓度的促性腺激素，以确定这些睾丸分泌睾酮的能力。当用羊促黄体生成素进行最大刺激时，五个物种的体外灌注睾丸分泌的睾酮量存在显著差异。五个物种的间质细胞质中滑面内质网、线粒体、粗面内质网和脂质的体积和表面密度也存在显著差异。最有趣的是，睾酮分泌与滑面内质网体积（r = 0.99）和表面（r = 0.99）密度之间呈线性正相关。因此，五个物种最大刺激睾丸分泌睾酮的差异几乎都可以由间质细胞滑面内质网数量的种间差异来解释。