Stoffel W, Krüger E, Melzner I
Hoppe Seylers Z Physiol Chem. 1980 May;361(5):773-9. doi: 10.1515/bchm2.1980.361.1.773.
During studies on the biosynthetic pathways of sphingomyelin, the condensation reaction between free fatty acids and sphingenine to ceramide catalysed in the reversed ceramidase reaction as proposed by Gatt (J. Biol. Chem. 238, PC 3131-3133 (1963) was reinvestigated. Radioactive long-chain fatty acids (palmitate and oleate) condense with ethanolamine, one of the buffer components of the original studies and yield N-acylethanolamine. N-Palmitoylethanolamine was isolated on a preparative scale from incubation mixtures and identified directly and also after derivatization by mass spectroscopy and comparison with the authentic synthetic compound. It is concluded that only the acyl-CoA-dependent ceramide synthesis is of biological significance.
在对鞘磷脂生物合成途径的研究中，重新研究了加特提出的（《生物化学杂志》238卷，PC 3131 - 3133页（1963年）），即游离脂肪酸与鞘氨醇在反向神经酰胺酶反应中催化生成神经酰胺的缩合反应。放射性长链脂肪酸（棕榈酸酯和油酸酯）与乙醇胺（原始研究中的缓冲成分之一）缩合，生成N - 酰基乙醇胺。通过制备规模从孵育混合物中分离出N - 棕榈酰乙醇胺，并直接鉴定，以及在衍生化后通过质谱分析并与真实合成化合物比较进行鉴定。得出的结论是，只有依赖酰基辅酶A的神经酰胺合成具有生物学意义。
