Nishi M
Arch Histol Jpn. 1978 Nov;41(5):411-26. doi: 10.1679/aohc1950.41.411.
The possible communication between the bile canaliculus and the space of Disse was examined by experimentally inducing obstructive jaundice in rats. Blood bilirubin levels were measured and the morphological changes in the liver were observed with the scanning electron microscope (SEM); Golgi's silver impregnation was also applied to demonstrate bile canaliculus. The normal bile canaliculus issued side branches but these ended blindly and showed no communication with the space of Disse. The pericapillary space of Disse frequently extended between the liver cells forming a paracapillary space. This space reached close to the bile canaliculus but never connected with it. Seven to 14 days after bile duct ligation, when a maximal level of bilirubin was reached, direct connection of bile canaliculi and pericapillary or paracapillary spaces could be found in many places. Two routes of bile leakage were discerned: One was formed by the side branch of the bile canaliculus directly connected to the pericapillary or paracapillary space, and the other was a communication of the laterally extended bile canaliculus to the paracapillary space, the junctional complex being overrun (Fig. 12).
通过在大鼠中实验性诱导梗阻性黄疸，研究了胆小管与狄氏间隙之间可能的连通性。测量血胆红素水平，并用扫描电子显微镜（SEM）观察肝脏的形态变化；还用高尔基银浸染法显示胆小管。正常胆小管发出侧支，但这些侧支末端呈盲端，与狄氏间隙无连通。狄氏毛细血管周围间隙经常在肝细胞之间延伸形成毛细血管旁间隙。这个间隙接近胆小管，但从未与之相连。胆管结扎后7至14天，当胆红素达到最高水平时，在许多部位可发现胆小管与毛细血管周围或毛细血管旁间隙直接相连。可识别出两条胆汁渗漏途径：一条由直接连接到毛细血管周围或毛细血管旁间隙的胆小管侧支形成，另一条是横向延伸的胆小管与毛细血管旁间隙连通，连接复合体被越过（图12）。
