Haymond M W, Howard C P, Miles J M, Gerich J E
J Chromatogr. 1980 Oct 10;183(4):403-9. doi: 10.1016/s0378-4347(00)81582-0.
A simple and reliable method is described for the determination of leucine flux in vivo using two stable isotopes of leucine and gas chromatography-mass spectrometry (GC-MS). [6,6,6-2H3]Leucine is administered as a primed-dose constant infusion in vivo and DL-[2H7]-leucine is added to plasma as an internal standard. Plasma leucine concentration and moles per cent enrichment of [2H3]leucine can be determined simultaneously by GC-MS and selected ion monitoring. Leucine flux calculated from the [6,6,6-2H3]leucine data was nearly identical to that obtained with L-[U-14C]leucine in dogs. This method is readily applicable to the study of leucine metabolism in humans of all ages and laboratory animals.
描述了一种简单可靠的方法，用于使用两种亮氨酸稳定同位素和气相色谱-质谱联用仪（GC-MS）测定体内亮氨酸通量。[6,6,6-2H3]亮氨酸在体内作为预充剂量持续输注给药，DL-[2H7]-亮氨酸作为内标添加到血浆中。血浆亮氨酸浓度和[2H3]亮氨酸的摩尔百分比丰度可通过GC-MS和选择离子监测同时测定。根据[6,6,6-2H3]亮氨酸数据计算出的亮氨酸通量与用L-[U-14C]亮氨酸在犬类中获得的通量几乎相同。该方法易于应用于所有年龄段的人类和实验动物的亮氨酸代谢研究。
