Metal chelate affinity chromatography. I. Influence of various parameters on the retention of nucleotides and related compounds.

The influence of various parameters, such as pH, ionic strength and temperature, on the retention of different nucleotides and related compounds on copper chelate gels has been investigated in order to understand the respective roles played by the different solute constituents (i.e., heterocyclic bases, sugars and phosphate groups) in the interaction and to define optimal conditions for subsequent application to the fractionation of oligo- and polynucleotides.