核苷酸通过磷酸钙沉淀。
Precipitation of nucleotides by calcium phosphate.
作者信息
Stenberg K, Oberg B, Chattopadhyaya J B
出版信息
Biochim Biophys Acta. 1982 May 31;697(2):170-3. doi: 10.1016/0167-4781(82)90073-2.
Earlier it has been shown that nucleic acids of high molecular weight can be be introduced into cells by coprecipitation with calcium phosphate. We have studied the requirements for calcium phosphate coprecipitation of shorter nucleotides. The degree of coprecipitation of dodecanucleotides lacking terminal phosphate varied between 25 and 72%. Tetramers with a 5'-monophosphate were coprecipitated to 29-87% by calcium phosphate. A high content of guanosine residues and an increased number of terminal phosphate groups increased the degree of coprecipitation of nucleotides. The trinucleotide pppA2'p5'A2'p5'A was effectively precipitated by calcium phosphate but the monophosphate and the core structure were not.
早些时候已经表明，高分子量核酸可通过与磷酸钙共沉淀的方式导入细胞。我们研究了较短核苷酸与磷酸钙共沉淀的条件。缺乏末端磷酸基团的十二聚体的共沉淀程度在25%至72%之间。带有5'-单磷酸的四聚体被磷酸钙共沉淀的比例为29%至87%。鸟苷残基的高含量以及末端磷酸基团数量的增加会提高核苷酸的共沉淀程度。三磷酸腺苷（pppA2'p5'A2'p5'A）能被磷酸钙有效沉淀，但单磷酸和核心结构则不能。
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