通过密度梯度离心法分离人自然杀伤细胞。
Isolation of human NK cells by density gradient centrifugation.
作者信息
Timonen T, Saksela E
出版信息
J Immunol Methods. 1980;36(3-4):285-91. doi: 10.1016/0022-1759(80)90133-7.
PMID:7430655
Abstract
Sedimentation characteristics of human NK cells in discontinuous Percoll density gradients were studied. NK activity against the leukaemic cell line K-562 peaked in a single low density fraction mainly consisting of large granular lymphocytes previously shown to be the principal NK cells in human peripheral blood. Percoll density gradient centrifugation provides a useful tool for analysis of human NK cells and their relationship to other blood mononuclear cells.
摘要
研究了人自然杀伤（NK）细胞在不连续聚蔗糖-泛影葡胺密度梯度中的沉降特性。针对白血病细胞系K-562的NK活性在一个单一的低密度组分中达到峰值，该组分主要由大颗粒淋巴细胞组成，此前已证明这些细胞是人类外周血中的主要NK细胞。聚蔗糖-泛影葡胺密度梯度离心为分析人NK细胞及其与其他血液单核细胞的关系提供了一种有用的工具。
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