Muramatsu T, Condamine H, Gachelin G, Jacob F
J Embryol Exp Morphol. 1980 Jun;57:25-36.
Six-day to 12-day mouse embryos were dissected and radiolabelled by culture in the presence of [3H]fucose. The radiolabelled embryos were extensively digested with Pronase. The resulting glycopeptides were analysed by Sephadex G-50 column chromatography. Glycopeptides from 6-day-old embryos were separated into two main peaks: one eluted near the excluded volume, the other in a well-retarded position. This elution profile was similar to that observed with glycopeptides prepared from embryonal carcinoma cells. The relative amount of the high-molecular-weight glycopeptides decreased during embryonic development and particularly around day 9. Glycopeptide elution profiles from 10-day embryos, or isolated organs of 12-day embryos, were indistinguishable from those obtained from differentiated teratocarcinoma-derived or adult cells. At least 30% of the large molecular weight glycopeptides appear to be located at the cell surface.
取6至12日龄的小鼠胚胎，在含有[3H]岩藻糖的培养基中进行解剖和放射性标记。将放射性标记的胚胎用链霉蛋白酶充分消化。所得糖肽通过葡聚糖G-50柱色谱进行分析。6日龄胚胎的糖肽被分离成两个主要峰：一个在排阻体积附近洗脱，另一个在滞留位置良好处洗脱。这种洗脱图谱与从胚胎癌细胞制备的糖肽所观察到的相似。高分子量糖肽的相对量在胚胎发育过程中，特别是在第9天左右减少。10日龄胚胎或12日龄胚胎的分离器官的糖肽洗脱图谱与从分化的畸胎癌衍生细胞或成年细胞获得的图谱无法区分。至少30%的大分子糖肽似乎位于细胞表面。
