[来自茄病镰刀菌-68的果聚糖酶]

[Levanase from Fusarium solani-68].

作者信息

Elisashvili V I, Loitsianskaia M S, Baranova T I, Bangura A

出版信息

Prikl Biokhim Mikrobiol. 1980 May-Jun;16(3):342-6.

Abstract

Extracellular levanase was isolated from the culture of Fusarium solani-68 by ammonium sulfate precipitation at 0.8 saturation and purified in part by Sephadex G-150 gel chromatography. Levanase showed maximum activity at pH = 6.0 and t = 45 degrees C. The levanase reaction (hydrolysis of high molecular levane with a molecular weight of 1--5 min) reached a maximum rate at a polysaccharide concentration of 6.7 mg/ml, Michaelis constant being 1.5 X 10(-6) M. Levanase remained stable at pH 5.4--7.5 and temperature 40--45 degrees C.

摘要

通过0.8饱和度的硫酸铵沉淀从茄病镰刀菌-68培养物中分离出胞外果聚糖酶，并通过Sephadex G-150凝胶色谱法进行部分纯化。果聚糖酶在pH = 6.0和t = 45℃时表现出最大活性。果聚糖酶反应（水解分子量为1 - 5分钟的高分子量果聚糖）在多糖浓度为6.7 mg/ml时达到最大速率，米氏常数为1.5×10(-6) M。果聚糖酶在pH 5.4 - 7.5和温度40 - 45℃下保持稳定。