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[采用改良的奥尔德里奇方法消除某些干扰人和动物血清中硫氰酸盐测定的氨基酸和蛋白质成分(作者译)]

[Use of a modified Aldridge method for the elimination of certain amino acids and protein components interfering with the determination of thiocyanate in human and animal sera (author's transl)].

作者信息

Thürkow B, Weuffen W

出版信息

Pharmazie. 1980 Aug;35(8):475-8.

PMID:7433496
Abstract

Since the amino acids and protein components remaining in the supernatant after protein precipitation react with bromine to give cyanogen bromide just as thiocyanate, they interfere with the determination of human and animal sera by the Aldridge method. This interference is widely eliminated by hydrochlorination of the amino acids or by reduction of the redox potential of bromine in an acid medium.

摘要

由于蛋白质沉淀后上清液中残留的氨基酸和蛋白质成分会像硫氰酸盐一样与溴反应生成溴化氰,它们会干扰用奥尔德里奇方法测定人和动物血清。通过氨基酸的盐酸化或在酸性介质中降低溴的氧化还原电位可广泛消除这种干扰。

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