Scolnik A J, Rubio M C, Caro R A, Ciscato V A
Biomedicine. 1978 Oct;28(5):274-7.
The histidine decarboxylase activity has been studied in the H-110 Lymphoma implanted in Balb-c mice. The enzymatic activity has been determined using DL-Histidine 1-14C by measuring the I--14CO2 liberated during the incubation. It has been found that the histidine decarboxylase activity in the tumor is the highest of the studied tissues. The pretreatment for 5 days with two cytostatic drugs, cyclophosphamide and 5-fluoruracile, determined a decrease of the enzymatic activity in the tumor of 33 and 54 per cent, respectively. This effect has not been observed in the lung. The pretreatment of the animals during 10 days with 0.1 mg per kg body of histamine, which in other experimental tumors induces an increase of the endogenous content of histamine, produces a decrease of the tumoral histidine decarboxylase activity. The histamine-14C uptake was not modified by this pretreatment. The subcellular localization of the radioactivity after pretreatment with histamine-14C evidenced that the nuclear fraction of the tumor contained between 2 and 10 times the radioactivity of other tissues of the same animal.
已对植入Balb-c小鼠体内的H - 110淋巴瘤中的组氨酸脱羧酶活性进行了研究。通过测量孵育过程中释放的I⁻¹⁴CO₂，使用DL - 组氨酸¹⁴C来测定酶活性。已发现肿瘤中的组氨酸脱羧酶活性在所研究的组织中是最高的。用两种细胞抑制药物环磷酰胺和5 - 氟尿嘧啶预处理5天，分别使肿瘤中的酶活性降低了33%和54%。在肺中未观察到这种效应。用每千克体重0.1毫克组胺对动物进行10天预处理，在其他实验性肿瘤中会诱导组胺内源性含量增加，但却使肿瘤组氨酸脱羧酶活性降低。这种预处理未改变组胺¹⁴C的摄取。用组胺¹⁴C预处理后放射性的亚细胞定位表明，肿瘤的核部分所含放射性是同一动物其他组织的2至10倍。
