Dressel T D, Goodale R L, Borner J W, Etani S
Ann Surg. 1980 Nov;192(5):614-9. doi: 10.1097/00000658-198011000-00005.
Previous work from this laboratory revealed in increased canine pancreatic intraductal pressure following cholinesterase inhibitor intoxication. The pressure was negatively correlated with serum butyrylcholinesterase (BChE) activity, suggesting that BChE activity mediated the pressure rise. This study uses a histochemical technique to investigate the tissue cholinesterase activity of the canine pancreatic sphincters and the effect of a cholinesterase inhibitor (ChEI) on tissue cholinesterase activity. In five control dogs, serial sections of the major and minor spincters were stained for acetylcholinesterase (AChE) and BChE activity. Four treated dogs were given the ChEI, O,O-diethyl-O- (2-isopropyl-6-methyl-4-pyrimidinyl) phosphoro-thioate, 25 mg/kg, one hour prior to excising the ampullae. In the control dogs, BChE activity is present in the periampullary nerves and the pancreatic smooth muscle sphincters. AChE activity is present in nerves but not in smooth muscle. In the treated group, following a dose of ChEI known to cause ductal hypertension, BChE activity was absent in the pancreatic sphincters but AChE activity was preserved in the periampullary nerves. These data suggest that the pancreatic ductal hypertension that occurs following ChEI administration is due to a selective reduction in pancreatic smooth muscle BChE activity.
该实验室之前的研究表明，胆碱酯酶抑制剂中毒后犬胰腺导管内压力会升高。这种压力与血清丁酰胆碱酯酶（BChE）活性呈负相关，提示BChE活性介导了压力升高。本研究采用组织化学技术来研究犬胰腺括约肌的组织胆碱酯酶活性以及胆碱酯酶抑制剂（ChEI）对组织胆碱酯酶活性的影响。对5只对照犬，对主、副括约肌的连续切片进行乙酰胆碱酯酶（AChE）和BChE活性染色。4只受试犬在切除壶腹前1小时给予ChEI，O,O-二乙基-O-（2-异丙基-6-甲基-4-嘧啶基）硫代磷酸酯，剂量为25mg/kg。在对照犬中，壶腹周围神经和胰腺平滑肌括约肌存在BChE活性。神经中存在AChE活性，但平滑肌中不存在。在受试组中，给予已知会导致导管高血压剂量的ChEI后，胰腺括约肌中不存在BChE活性，但壶腹周围神经中的AChE活性得以保留。这些数据表明，ChEI给药后发生的胰腺导管高血压是由于胰腺平滑肌BChE活性选择性降低所致。
