一种从淋巴样细胞系悬浮培养物中去除无活力细胞的快速高效方法。
A rapid and efficient procedure for the removal of nonviable cells from suspension culture of lymphoid cell lines.
作者信息
Hurrell J G, Black P H, Haber E, Zurawski V R
出版信息
Aust J Exp Biol Med Sci. 1980 Aug;58(4):373-6. doi: 10.1038/icb.1980.36.
PMID:7436883
Abstract
The selective removal of nonviable cells from suspension cultures of lymphoid cell lines by buoyant density centrifugation at 400 x g on a Ficoll-Hypaque (FH) cushion is described. Viable cells collect at the medium-FH interface whereas nonviable cells pellet. This method has proven useful in separating viable from nonviable cells with human lymphoblastoid cell lines, rabbit lymphoid cell lines and mouse myeloma and hybridoma cell lines.
摘要
本文描述了通过在菲可-泛影葡胺（FH）垫层上以400×g进行浮力密度离心，从淋巴样细胞系的悬浮培养物中选择性去除无活力细胞的方法。有活力的细胞聚集在培养基-FH界面，而无活力的细胞形成沉淀。该方法已被证明可用于从人淋巴母细胞系、兔淋巴样细胞系以及小鼠骨髓瘤和杂交瘤细胞系中分离有活力和无活力的细胞。
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