Etheridge J R, Lloyd L C
Aust Vet J. 1980 Mar;56(3):101-5. doi: 10.1111/j.1751-0813.1980.tb05644.x.
Complement-fixing antibody to Mycoplasma hyopneumoniae in the serums of pigs experimentally infected with enzootic pneumonia was demonstrated by comparing the haemolytic titre of guinea-pig complement titrated in the presence of heated test serum, M. hyopneumoniae antigen and unheated normal pig serum with the titre obtained when the antigen was omitted. The haemolytic titres against sensitised sheep erythrocytes were determined after a fixation period of 16 to 18 hours at 5 degrees C. When serums, collected at intervals of 3 to 7 days, from 43 pigs exposed to pigs experimentally infected with enzootic pneumonia were tested, 4.6 or more complement units were first fixed 14 to 44 (mean 23.4) days after contact began. Serums collected subsequently fixed from 4.6 to more than 31 complement units. This positive reaction usually persisted until the pigs were killed 4 to 35 weeks after contact began. Thirty-three had gross enzootic pneumonia lesions and 9 had lung lesions detected microscopically. Serum antibody was not detected in 73 weaned pigs aged 7 weeks in a pneumonia-free herd but serums from 9 of 15 unweaned piglets aged 9 to 14 days in the same herd, fixed between 3 and 7 complement units.
通过比较在加热的试验血清、猪肺炎支原体抗原和未加热的正常猪血清存在的情况下滴定的豚鼠补体的溶血效价与省略抗原时获得的效价，证明了实验感染地方性肺炎的猪血清中针对猪肺炎支原体的补体结合抗体。在5℃下固定16至18小时后，测定针对致敏绵羊红细胞的溶血效价。当检测从43头接触实验感染地方性肺炎的猪的猪身上每隔3至7天采集的血清时，在接触开始后14至44天（平均23.4天）首次固定4.6个或更多补体单位。随后采集的血清固定了4.6至超过31个补体单位。这种阳性反应通常持续到接触开始后4至35周猪被宰杀。33头有明显的地方性肺炎病变，9头在显微镜下检测到肺部病变。在一个无肺炎猪群中，73头7周龄断奶仔猪未检测到血清抗体，但同一猪群中15头9至14日龄未断奶仔猪中的9头血清固定了3至7个补体单位。
