Tantawi H H, Al-Sheikhly F
Avian Dis. 1980 Apr-Jun;24(2):455-62.
Two strains of pigeon herpes encephalomyelitis virus BVC 78, a virulent field strain, and BVC 78 T7, a cell-culture-adapted strain, were assayed in embryonated chicken eggs and in chicken-embryo-fibroblast (CEF) cell culture to investigate their cytopathogenicity, growth kinetics, plaque characters, and virulence. In CEF the BVC 78 induced syncytia 24-48 hr postinoculation (PI), while the BVC 78 T7 induced only cell rounding 72 hr PI. The strains differed in growth patterns in CEF. The BVC 78 and BVC 78 T7 strains had respective logarithmic phases at 24 and 72 hr PI and maximum virus yields at 36 and 120 hr PI. The BVC 78 T7 was more cell-associated than the BVC 78, the ratio of cell-bound to cell-free virus was about 2 for the former and near unity for the latter. In CEF the BVC 78 induced plaques 1-2 mm in diameter by the third day PI, while the BVC 78 T7 produced small plaques 0.3-0.5 mm in diameter by the 5th day PI. The BVC T7 strain was of low killing capacity for chicken embryos, in contrast to the high killing capacity of the BVC 78. Pigeons inoculated subcutaneously with the former strain and challenged 21 days later with the virulent strain proved resistant.
对两株鸽疱疹性脑脊髓炎病毒BVC 78（一种强毒野毒株）和BVC 78 T7（一种细胞培养适应株）在鸡胚和鸡胚成纤维细胞（CEF）培养物中进行了检测，以研究它们的细胞致病性、生长动力学、蚀斑特征和毒力。在CEF中，BVC 78在接种后（PI）24 - 48小时诱导形成合胞体，而BVC 78 T7在PI 72小时仅诱导细胞变圆。这两个毒株在CEF中的生长模式不同。BVC 78和BVC 78 T7毒株分别在PI 24小时和72小时进入对数生长期，在PI 36小时和120小时达到最大病毒产量。BVC 78 T7比BVC 78与细胞的结合性更强，前者细胞结合病毒与游离病毒的比例约为2，后者接近1。在CEF中，BVC 78在PI第三天诱导出直径为1 - 2毫米的蚀斑，而BVC 78 T7在PI第5天产生直径为0.3 - 0.5毫米的小蚀斑。与BVC 78的高致死能力相比，BVC T7毒株对鸡胚的致死能力较低。皮下接种前一种毒株的鸽子在21天后用强毒株攻击时被证明具有抗性。
