Giesen J, Sondermann M, Juengling E, Kammermeier H
Basic Res Cardiol. 1980 Jul-Aug;75(4):515-25. doi: 10.1007/BF01907833.
In isolated perfused rat hearts (medium: Krebs-Ringer solution containing about 15% bovine red cells) the following parameters were estimated: heart rate (F), left intraventricular peak pressure (P), dP/dt, oxygen consumption (VO2); myocardial tissue content of glycogen, ATP, ADP, AMP, cAMP, phosphocreatin (PC) and inorganic phosphate (Pi). Isoproterenol (ISO) was administered to the non circulating system in the concentration range of 5 x 10(-10) to 5 x 10(-5) M for 4 min and 1 hour respectively by infusion into the perfusate close to the aortic canula. After administration period of 4 min dependent on the concentration of ISO, P, dP/dt, VO2 and the content of cAMP are increased. The ratios of PC/Pi and ATP/ADP as well as glycogen content are reduced. For an administration period of 1 hour at a level of 5 x 10(-9) M the effects of isoproterenol are maintained. At ISO concentration higher than 5 x 10(-9) M the effects on mechanical parameters and VO2 fall to the level of those values which are produced at 5 x 10(-9) M ISO. In contrast, high energy phosphates and glycogen content remain reduced while that of cAMP is elevated.
在离体灌注大鼠心脏（介质：含约15%牛红细胞的 Krebs-Ringer 溶液）中，测定了以下参数：心率（F）、左心室内峰压（P）、dP/dt、耗氧量（VO₂）；心肌组织中糖原、三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）、环磷酸腺苷（cAMP）、磷酸肌酸（PC）和无机磷酸盐（Pi）的含量。分别以5×10⁻¹⁰至5×10⁻⁵ M 的浓度，通过向靠近主动脉插管处的灌注液中输注异丙肾上腺素（ISO）4分钟和1小时，将其给予非循环系统。给药4分钟后，根据 ISO 的浓度，P、dP/dt、VO₂和 cAMP 的含量增加。PC/Pi 和 ATP/ADP 的比值以及糖原含量降低。在5×10⁻⁹ M 的水平给药1小时，异丙肾上腺素的作用得以维持。当 ISO 浓度高于5×10⁻⁹ M 时，对机械参数和 VO₂的作用降至在5×10⁻⁹ M ISO 时产生的水平。相反，高能磷酸盐和糖原含量仍然降低，而 cAMP 的含量升高。
