Forkmann G, Dangelmayr B
Biochem Genet. 1980 Jun;18(5-6):519-27. doi: 10.1007/BF00484399.
In flowers of Dianthus caryophyllus (carnation), the gene I is concerned with a discrete step in flavonoid biosynthesis, Genotypes with recessive (ii) alleles produce yellow flowers, which contain the chalcone isosalipurposide (naringenin-chalcone-2'-glucoside) as the major petal pigment, but in genotypes with wild-type alleles flavonols and anthocyanins can be formed and the flowers are white or red. Enzymatic measurements on petal extracts of four strains with different flower coloration revealed a clear correlation between accumulation of chalcone in recessive genotypes and deficiency of chalcone isomerase (E.C. 5.5.1.6) activity. From the chemogenetic and enzymological evidence it can be concluded that naringenin-chalcone is the first product of the synthesis of the flavonoid skeleton and that only the conversion of naringenin-chalcone to naringenin furnishes the substrate for the further reactions to flavonol and anthocyanin.
在石竹（康乃馨）的花朵中，基因I参与类黄酮生物合成的一个离散步骤。具有隐性（ii）等位基因的基因型产生黄色花朵，其主要花瓣色素为查耳酮异水苏苷（柚皮素查耳酮-2'-葡萄糖苷），但在具有野生型等位基因的基因型中，可以形成黄酮醇和花青素，花朵为白色或红色。对四种不同花色菌株的花瓣提取物进行的酶活性测定表明，隐性基因型中查耳酮的积累与查耳酮异构酶（E.C. 5.5.1.6）活性的缺乏之间存在明显的相关性。从化学遗传学和酶学证据可以得出结论，柚皮素查耳酮是类黄酮骨架合成的第一个产物，只有柚皮素查耳酮向柚皮素的转化才为黄酮醇和花青素的进一步反应提供底物。
