Verweij H, van Steveninck J
Biochim Biophys Acta. 1980 Nov 18;602(3):591-9. doi: 10.1016/0005-2736(80)90337-5.
Semicarbazide can interfere with oxidative processes in the red blood cell membrane via different modes of action. Treatment of human red blood cell membranes with O3, results, among other effects, in cross-linking of membrane proteins and inhibition of glyceraldehyde-3-phosphate dehydrogenase activity. Semicarbazide inhibits these effects by acting as an O3 scavenger. The effect of semicarbazide as an O3 scavenger is complicated by the fact that ozonolysis of semicarbazide yields a product that causes inhibition of glyceraldehyde-3-phosphate dehydrogenase. Glyceraldehyde-3-phosphate dehydrogenase inhibition can also be provoked by incubation of membrane suspensions with O3-treated phospholipids. Semicarbazide prevented this effect by interaction with an inhibitory O3-phospholipid reaction product. Protoporphyrin-induced photodynamic cross-linking of membrane proteins is chemically distinct from O3-induced cross-linking. Photodynamic cross-linking is also inhibited by semicarbazide, in this case via reaction with a histidine photooxidation product.
氨基脲可通过不同作用方式干扰红细胞膜中的氧化过程。用O3处理人红细胞膜，除其他作用外，会导致膜蛋白交联并抑制甘油醛-3-磷酸脱氢酶活性。氨基脲通过作为O3清除剂来抑制这些作用。氨基脲作为O3清除剂的作用因以下事实而变得复杂：氨基脲的臭氧分解产生一种会抑制甘油醛-3-磷酸脱氢酶的产物。用O3处理过的磷脂孵育膜悬浮液也可引发甘油醛-3-磷酸脱氢酶抑制。氨基脲通过与抑制性的O3-磷脂反应产物相互作用来防止这种作用。原卟啉诱导的膜蛋白光动力交联在化学上与O3诱导的交联不同。光动力交联也受到氨基脲的抑制，在这种情况下是通过与组氨酸光氧化产物反应来实现的。
