Crabb W D, Firshein W, Infante A A
Biochim Biophys Acta. 1980 Oct 17;609(3):456-63. doi: 10.1016/0005-2787(80)90119-7.
A complex containing deoxyribonucleic acid, protein and lipid has been isolated from the nuclei of the sea urchin Stronglocentrotus pupuratus by gentle lysis of nuclei and centrifugation of the lysate in a renografin gradient. The complex is similar in several respects to DNA-membrane complexes isolated by the sarkosyl M-band technique [1]. These include the following: 1, most of the cellular DNA is present in the complex but the bulk of the DNA may be removed by sonication or nuclease treatment; 2, nascent DNA is preferentially found in the complex; and 3, destruction of membrane/protein components removes DNA found in the complex.
