Ponka P, Borova J, Neuwirt J
Biochim Biophys Acta. 1980 Nov 3;632(4):527-35. doi: 10.1016/0304-4165(80)90329-3.
A high level of non-heme iron (either labelled or unlabelled) in mitochondria, ferritin and low-molecular-weight pool of reticulocytes was induced by preincubation with isonicotinic acid hydrazide or penicillamine together with either 59Fe- of 56Fe-labelled transferrin. Addition of apotransferrin during reincubation of 59Fe-labelled reticulocytes was accompanied by the transfer of 59Fe from low-molecular-weight pool to transferrin, which was found in the reticulocyte cytosol both free and bound to a carrier. Similarly, when cells were reincubated with 125I-labelled transferrin, more 125I-labelled radioactivity was found, in both free and carrier-bound transferrin peaks, in reticulocytes with a high level of low-molecular-weight cold iron than in control ones. These results suggest that transferrin enters reticulocytes and takes up iron from low-molecular-weight pool.
用异烟肼或青霉胺与59Fe或56Fe标记的转铁蛋白一起预孵育，可诱导线粒体、铁蛋白和网织红细胞低分子量池中的非血红素铁（标记的或未标记的）水平升高。在重新孵育59Fe标记的网织红细胞期间加入脱铁转铁蛋白，伴随着59Fe从低分子量池转移到转铁蛋白，在网织红细胞胞质溶胶中发现转铁蛋白既游离又与载体结合。同样，当细胞与125I标记的转铁蛋白重新孵育时，与对照细胞相比，低分子量冷铁水平高的网织红细胞中，游离和载体结合的转铁蛋白峰中均发现更多的125I标记放射性。这些结果表明转铁蛋白进入网织红细胞并从低分子量池中摄取铁。
