线虫类小杆线虫X染色体的研究：甲基磺酸乙酯诱导的可见突变。

Studies on the X-chromosome of the nematode Panagrellus redivivus: EMS induced visible mutations.

作者信息

Burke D J, Samoiloff M R

出版信息

Can J Genet Cytol. 1980;22(2):295-302. doi: 10.1139/g80-035.

DOI:10.1139/g80-035

Abstract

Young virgin males and females of the free-living nematode Panagrellus redivivus (deMan 1913) Goodey 1945 were mutagenized with 0.05 M ethyl methanesulphonate for 4 h. Twenty-four X-linked mutants were isolated, but only eight could be maintained as homozygotes due to severity of phenotype and mating inefficiency. The mutants define six complementation groups. The mutation frequency per locus was estimated to be 4.4 X 10(-4) following EMS treatment. An estimated 330 essential loci occur on the X-chromosome of P. redivivus.

摘要

自由生活的线虫——食菌茎线虫（Panagrellus redivivus，（德曼，1913年）古迪，1945年）的年轻未交配雌雄个体，用0.05 M甲磺酸乙酯诱变4小时。分离出24个X连锁突变体，但由于表型严重和交配效率低下，只有8个能维持为纯合子。这些突变体定义了六个互补群。经EMS处理后，每个基因座的突变频率估计为4.4×10⁻⁴。据估计，食菌茎线虫的X染色体上有330个必需基因座。

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