用莱文方法分析荧光猝灭数据。
Analysis of fluorescence quenching data by the method of Levine.
作者信息
Parsons D L
出版信息
Clin Chem. 1980 Dec;26(13):1869-71.
The quenching of intrinsic protein fluorescence upon binding of ligand is a relatively quick and easy method for studying these interactions. The method of Steiner et al. (J. Biol. Chem. 241: 560, 1966) and the method of Levine (Clin. Chem. 23: 2292, 1977) have been used by different authors to interpret the data obtained from fluorescence-quenching studies. However, as is shown here, the method of Levine is not strictly valid when the protein contains more than one binding site for the ligand. Therefore, this method only approximates binding constants from such data, with an accuracy that depends on the specific binding properties of the interactants.
配体结合时蛋白质固有荧光的猝灭是研究这些相互作用的一种相对快速且简便的方法。不同作者采用了施泰纳等人（《生物化学杂志》241: 560, 1966）的方法以及莱文（《临床化学》23: 2292, 1977）的方法来解释从荧光猝灭研究中获得的数据。然而，如下所示，当蛋白质对配体含有多个结合位点时，莱文的方法并不严格有效。因此，该方法只能从这些数据近似得出结合常数，其准确性取决于相互作用物的特定结合特性。
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