Rydlander L, Pigon A, Edström J E
Chromosoma. 1980;81(1):101-13. doi: 10.1007/BF00292425.
The 75S RNA originating in the large Balbiani rings 1 and 2 (BRI and 2) was isolated and used for in vitro translation in the mRNA dependent reticulocyte lystate. Conditions (K+-concentration, temperature, time etc). were optimized for obtaining translation products of maximal size. Polypeptide chains up to about 500,000 D were obtained but no complete translation products. Trypic fingerprints were performed on the in vitro products as well as on the secretory protein components nos. I and II+III labelled with 35S-methionine. There was a large degree of correspondence between the fingerprint of the in vitro product and that of component I but less to that of component II+III. The results suggest that 75S RNA with an origin in the BR1 and BR2 codes for the giant secretory protein component I.
从大型巴尔比亚尼环1和2（BR1和BR2）中分离出75S RNA，并将其用于依赖mRNA的网织红细胞裂解物中的体外翻译。对条件（钾离子浓度、温度、时间等）进行了优化，以获得最大尺寸的翻译产物。获得了分子量高达约500,000道尔顿的多肽链，但没有完整的翻译产物。对体外产物以及用35S-甲硫氨酸标记的分泌蛋白成分I和II+III进行了胰蛋白酶指纹图谱分析。体外产物的指纹图谱与成分I的指纹图谱有很大程度的对应，但与成分II+III的指纹图谱对应程度较低。结果表明，起源于BR1和BR2的75S RNA编码巨大的分泌蛋白成分I。
