Díez J L, Santa-Cruz M C, Villanueva A, Aller P
Chromosoma. 1980;81(2):263-9. doi: 10.1007/BF00285952.
The possible dependence of puffing of the Balbiani rings (BRs) on the protein synthesis has been investigated by studying the response of these structures to protein synthesis inhibition induced by cycloheximide and anisomycin. When larvae of Chironomus thummi belonging to middle IV instar (BR1 repressed, BR2 expanded) are subjected to short treatment (3--6 h) with these drugs, BR1 and BR2 puffing states remain essentially unaffected. But when the same treatments are applied to galactose-pretreated larvae (BR1 expanded, BR2 repressed), selective reactivation of the collapsed BR2 occurs. These observations suggest that maintenance of a given puffing state can be dependent, to a variable extent, on the supply of newly synthesized proteins. In particular, selective re-expansion of galactose-repressed BR2 induced by the drugs seem to indicate the existence of repressor-like factor whose activity would be triggered by the galactose treatment.
通过研究巴尔比亚尼环（BRs）对环己酰亚胺和茴香霉素诱导的蛋白质合成抑制的反应，已对BRs的膨胀与蛋白质合成之间可能存在的依赖性进行了研究。当处于IV龄中期（BR1受抑制，BR2膨胀）的摇蚊幼虫用这些药物进行短时间处理（3 - 6小时）时，BR1和BR2的膨胀状态基本不受影响。但是，当对用半乳糖预处理过的幼虫（BR1膨胀，BR2受抑制）进行相同处理时，塌陷的BR2会发生选择性重新激活。这些观察结果表明，特定膨胀状态的维持在不同程度上可能依赖于新合成蛋白质的供应。特别是，药物诱导的半乳糖抑制的BR2的选择性重新膨胀似乎表明存在一种阻遏物样因子，其活性会被半乳糖处理所触发。
