Arai T, Takahashi K, Ishiguro K, Yazawa K
J Antibiot (Tokyo). 1980 Sep;33(9):951-60. doi: 10.7164/antibiotics.33.951.
This paper reports on an increased production of saframycin A, which is a satellite antibiotic to streptothricin and a trace component in the culture of Streptomyces lavendulae No. 314. Some improvement in the yield of the antibiotic was attained by supplementing the standard medium with two end-products of metabolic pathways related to saframycin biosynthesis. A further significant increase of saframycin A potency could be attained by addition of NaCN to the culture broth. Finally, a drastic degradation of saframycin A in the culture was successfully prevented by maintaining the pH lower than 5.5 after peak production of the antibiotic. The combined application of these improvements resulted in approximately 1,000-fold increase in saframycin A production as compared with the parental level. The precursor of saframycin A in the culture, which yielded saframycin A upon cyanation was isolated, characterized and named saframycin S. Physicochemical properties of saframycin S were described and structural formulae were discussed.
本文报道了番红菌素A产量的增加，番红菌素A是链丝菌素的一种附属抗生素，也是薰衣草链霉菌314号菌株培养物中的微量成分。通过在标准培养基中添加与番红菌素生物合成相关的代谢途径的两种终产物，抗生素的产量得到了一定提高。向培养液中添加NaCN可使番红菌素A的效价进一步显著提高。最后，通过在抗生素产量达到峰值后将pH维持在5.5以下，成功防止了培养液中番红菌素A的剧烈降解。这些改进措施的联合应用使番红菌素A的产量比亲本水平提高了约1000倍。分离并鉴定了培养物中经氰化后产生番红菌素A的番红菌素A前体，并将其命名为番红菌素S。描述了番红菌素S的物理化学性质并讨论了其结构式。
