Segerson E C, Ganapathy S N
J Anim Sci. 1980 Aug;51(2):386-94. doi: 10.2527/jas1980.512386x.
Studies were conducted to evaluate uterine motility and fertility of ova in ewes either given or not given supplemental selenium/vitamin E (SSE or NSE, respectively). The ewes were maintained on either an adequate plane of nutrition (APN) or an inadequate plane of nutrition (IPN). In a fertility study, 60 ewes were allotted to four treatment groups, (15 ewes per group): SSE-APN, NSE-APN, SSE-IPN and NSE-IPN. A 2-ml injection, containing 10 mg of Se as selenite and 136 IU of vitamin E as alpha-tocopheryl acetate, was given IM to designated ewes (SSE) at 21-day intervals. NSE ewes were given injection of corn oil. After 150 days of SSE supplementation, ewes were checked for estrus, bred to rams and laparotomized approximately, 72 hr after the onset of estrus for determination of fertility of ova. The proportion of recovered ova that were fertilized was, for each treatment: SSE-APN (19 of 19), NSE-APN (17 of 22), SSE-IPN (five of 10) and NSE-IPN (six of 14). Fertility of ova was affected (P < .005) by plane of nutrition and tended to be affected (P < .1) by Se/vitamin E supplementation. Throughout the fertility study, ewes in the APN group maintained their body weight, while ewes in the IPN groups lost .075 kg/ewe/day. In a uterine contraction study, ewes in the SSE-APN and NSE-APN groups were laparotomized at the onset of estrus for quantitation of the directional pattern of uterine contractions. The number of total contractions for SSE-APN ewes and NSE-APN ewes during a 10-min interval was 42.7 +/- 3.5 and 33.2 +/- 2.6 (P < .05), respectively, and number of contractions moving toward the oviduct was 21.0 +/- 2.8 and 13.4 +/- 1.2 (P < .025), respectively. Se was greater (P < .001) in serum of SSE ewes than in serum of NSE ewes, while vitamin E was greater in serum of SSE-APN ewes than in that of both groups of NSE ewes.
开展了多项研究，以评估分别给予或未给予补充硒/维生素E（分别为SSE或NSE）的母羊的子宫活动能力和卵子的受精能力。母羊分别维持在充足营养水平（APN）或不足营养水平（IPN）。在一项受精能力研究中，60只母羊被分配到四个处理组（每组15只母羊）：SSE - APN、NSE - APN、SSE - IPN和NSE - IPN。给指定的母羊（SSE）每隔21天肌肉注射一次2毫升注射液，其中含10毫克亚硒酸盐形式的硒和136国际单位醋酸生育酚形式的维生素E。NSE组母羊注射玉米油。在补充SSE 150天后，检查母羊是否发情，与公羊交配，并在发情开始后约72小时进行剖腹手术，以确定卵子的受精能力。各处理组中已受精的回收卵子比例分别为：SSE - APN组（19个中的19个）、NSE - APN组（22个中的17个）、SSE - IPN组（10个中的5个）和NSE - IPN组（14个中的6个）。卵子的受精能力受营养水平影响（P <.005），并且有受硒/维生素E补充影响的趋势（P <.1）。在整个受精能力研究过程中，APN组的母羊维持了体重，而IPN组的母羊每只每天体重减轻0.075千克。在一项子宫收缩研究中，在发情开始时对SSE - APN组和NSE - APN组的母羊进行剖腹手术，以定量子宫收缩的方向模式。SSE - APN组母羊和NSE - APN组母羊在10分钟间隔内的总收缩次数分别为42.7±3.5和33.2±2.6（P <.05），向输卵管方向移动的收缩次数分别为21.0±2.8和13.4±1.2（P <.025）。SSE组母羊血清中的硒含量高于NSE组母羊（P <.001），而SSE - APN组母羊血清中的维生素E含量高于两组NSE组母羊。
