Calvert C C, Smith L W
J Anim Sci. 1980 Aug;51(2):414-21. doi: 10.2527/jas1980.512414x.
Arsenic from two sources--arsanilic acid and 3-nitro-4-hydroxyphenylarsonic acid (3-nitro)--was fed to mature wethers and lactating dairy cows, and the uptake and depletion of arsenic in tissues and milk were determined. In experiments with sheep, arsenic concentration in liver, kidney and blood increased as the level of arsenic from arsanilic acid and 3-nitro was increased in feed. Muscle arsenic was relatively unaffected by the level of arsenic in feed from either source. No statistically significant differences in arsenic levels were observed between liver and kidney tissue, over all levels fed, or for either source of arsenic. When comparisons were made within each tissue, arsenic levels were found to be significantly lower in sheep fed 3-nitro. This difference between 3-nitro and arsanilic acid within tissues was not observed in muscle or blood. The depletion rates for arsenic were the same for all tissues, and the time required for tissue to return to pretreatment arsenic levels was dependent on the initial tissue concentration. Within 6 days of removal of arsenic from the sheep diets, 85% of the arsenic in the liver of sheep fed arsanilic acid and 81% of the arsenic in the liver of sheep fed 3-nitro was depleted. Arsenic levels in the kidneys of sheep fed arsanilic acid and 3-nitro were depleted by 87 and 83%, respectively. In the experiments with lactating dairy cows, significantly higher levels of arsenic in milk were observed for cows fed either 3.2 or 4.8 mg of arsenic per kilogram of body weight from arsanilic acid or 3-nitro. In the experiment in which 3-nitro was fed at a level of 4.8 mg/kg of body weight for 28 days, arsenic levels plateaued after 14 days of feeding and remained constant for the next 14 days. Milk arsenic from 3-nitro returned to pre-experiment levels within 5 days after arsenic was removed from the feed. In the experiment conducted with arsanilic acid, arsenic feeding was stopped after 5 days and milk arsenic levels returned to pre-experiment levels within 7 days. Because of the short duration of this study, no plateauing effect was observed.
将两种来源的砷——阿散酸和3-硝基-4-羟基苯胂酸（3-硝基）——喂给成年阉羊和泌乳奶牛，并测定组织和牛奶中砷的摄取和消耗情况。在绵羊实验中，随着饲料中阿散酸和3-硝基砷含量的增加，肝脏、肾脏和血液中的砷浓度也随之升高。肌肉中的砷含量相对不受任何一种来源饲料中砷含量的影响。在所有投喂水平下，或者对于任何一种砷来源，肝脏和肾脏组织中的砷含量均未观察到统计学上的显著差异。当在每个组织内进行比较时，发现投喂3-硝基的绵羊体内的砷含量显著较低。在肌肉或血液中未观察到组织内3-硝基和阿散酸之间的这种差异。所有组织中砷的消耗率相同，组织恢复到预处理砷水平所需的时间取决于初始组织浓度。在从绵羊日粮中去除砷后的6天内，投喂阿散酸的绵羊肝脏中85%的砷和投喂3-硝基的绵羊肝脏中81%的砷被消耗。投喂阿散酸和3-硝基的绵羊肾脏中的砷含量分别消耗了87%和83%。在泌乳奶牛实验中，投喂每千克体重3.2毫克或4.8毫克阿散酸或3-硝基砷的奶牛，牛奶中的砷含量显著更高。在以每千克体重4.8毫克的水平投喂3-硝基28天的实验中，投喂14天后砷含量达到平稳状态，并在接下来的14天内保持不变。从饲料中去除砷后，3-硝基的牛奶砷含量在5天内恢复到实验前水平。在用阿散酸进行的实验中，5天后停止投喂砷，牛奶砷含量在7天内恢复到实验前水平。由于本研究持续时间较短，未观察到平稳效应。
