Ong T, Mukhtar M, Wolf C R, Zeiger E
J Environ Pathol Toxicol. 1980 Aug;4(1):55-65.
Studies have been conducted to determine aryl hydrocarbon hydroxylase, benzphetamine-N-demethylase, epoxide hydrase and glutathione S-transferase activities and cytochrome P-450 content in the liver 9000xg supernatant fractions from rats treated with either phenobarbital, beta-naphthoflavone, ARoclor 1254 or a combination of phenobarbital and beta-naphthoflavone. The metabolic activation of 2-anthramine, 2-acetylaminofluorene, 3-methylcholanthrene, and benzo(a)pyrene to metabolites that are mutagenic in Salmonella typhimurium TA1535 or TA98 by S-9 from rats treated with these inducers was also determined. The induction of drug metabolizing enzymes in the S-9 from Aroclor or phenobarbital plus beta-naphthoflavone treated animals was very similar. The overall results seem to indicate that a combination treatment of phenobarbital and beta-naphthoflavone can be used as a substitute for Aroclor 1254 as inducer for enzyme activity and for the in vitro activation of promutagens to mutagenic metabolites in Salmonella mutagenesis assay systems.
已开展研究以测定用苯巴比妥、β-萘黄酮、多氯联苯混合物Aroclor 1254或苯巴比妥与β-萘黄酮的组合处理的大鼠肝脏9000xg上清液组分中的芳烃羟化酶、苄非他明-N-脱甲基酶、环氧化物水合酶和谷胱甘肽S-转移酶活性以及细胞色素P-450含量。还测定了用这些诱导剂处理的大鼠的S-9将2-氨基蒽、2-乙酰氨基芴、3-甲基胆蒽和苯并(a)芘代谢活化为在鼠伤寒沙门氏菌TA1535或TA98中具有致突变性的代谢物的情况。来自用Aroclor或苯巴比妥加β-萘黄酮处理的动物的S-9中药物代谢酶的诱导情况非常相似。总体结果似乎表明，苯巴比妥和β-萘黄酮的联合处理可作为Aroclor 1254的替代品，用于酶活性诱导以及在沙门氏菌诱变试验系统中将前诱变剂体外活化为致突变性代谢物。
