Lehtonen E
J Embryol Exp Morphol. 1980 Aug;58:231-49.
The cleavage behavior of cells isolated from 1- to 8-cell-stage mouse embryos was studied with time-lapse video equipment; changes in cellular dimensions and their timing were recorded. The division of an isolated cell results in the formation of a twin-cell pair. The divisions of these two cells were always asynchronous. In each division the volume of a daughter cell was approximately half of that of the parental cell but its apparent surface area was 59-65% of that of the parental cell. Consequently, the ratio of apparent surface area to volume increased in each division by 25-30%. The most noticeable changes were observed in the relationship between the two daughter cells of each division. After cytokinesis, the intercellular contact area gradually increased during the following cell cycle in the 2/8- and 2/16-cell pairs, whereas it hardly changed in the 2/2- and 2/4-cell pairs. The comparison of the behaviour of the daughter cells on different substrates suggested that the zona pellucida and the mid body might have a role in the contact development at the early stages. Scanning electron microscopy was used for studying changes in the density of cell surface microvilli in an attempt to explain how the cells regulate their intercellular contacts.
利用延时视频设备研究了从1至8细胞期小鼠胚胎分离出的细胞的分裂行为；记录了细胞尺寸的变化及其时间。单个分离细胞的分裂会形成一对双细胞。这两个细胞的分裂总是不同步的。在每次分裂中，子细胞的体积约为亲代细胞的一半，但其表观表面积为亲代细胞的59 - 65%。因此，每次分裂中表观表面积与体积的比值增加了25 - 30%。在每次分裂的两个子细胞之间的关系中观察到最明显的变化。胞质分裂后，在接下来的细胞周期中，2/8和2/16细胞对中的细胞间接触面积逐渐增加，而在2/2和2/4细胞对中几乎没有变化。对不同底物上子细胞行为的比较表明，透明带和中体可能在早期接触发育中起作用。使用扫描电子显微镜研究细胞表面微绒毛密度的变化，试图解释细胞如何调节它们的细胞间接触。
