Jones D H, Gray E G, Barron J
J Neurocytol. 1980 Aug;9(4):493-504. doi: 10.1007/BF01204838.
Microtubules were shown to remain intact in brain slices and subfractions maintained at 0 degrees C for 1 h. Under the same conditions, microtubules isolated from brain by warm assembly-cold disassembly methods, disassemble into their constituent subunit proteins. No selective depletions of microtubules were seen when brain slices were incubated in homogenizing buffer at either 0 degrees C or 37 degrees C. The response of native microtubules in brain slices in incubation in other solutions showed that their properties were otherwise the same as those of assembled microtubules. The separated alpha and beta subunits of isolated cold labile and cold stable microtubules were compared by electrophoresis and isoelectric focusing and were shown to possess the same mobilities. The results suggest that native microtubules are temperature insensitive and that isolated microtubules are assembled from pre-existing pools of subunit proteins. The results further suggest that native microtubules possess a factor, lacking in isolated assembled microtubules, which confers temperature stability on the former.
研究表明，微管在0摄氏度下保存1小时的脑切片和亚组分中保持完整。在相同条件下，通过温育组装-冷拆卸方法从脑中分离的微管会分解成其组成亚基蛋白。当脑切片在0摄氏度或37摄氏度的匀浆缓冲液中孵育时，未观察到微管的选择性消耗。脑切片中天然微管在其他溶液中孵育的反应表明，它们的性质与组装微管的性质相同。通过电泳和等电聚焦比较了分离的冷不稳定和冷稳定微管的α和β亚基，结果显示它们具有相同的迁移率。结果表明，天然微管对温度不敏感，分离的微管由预先存在的亚基蛋白池组装而成。结果进一步表明，天然微管具有一种在分离的组装微管中缺乏的因子，该因子赋予前者温度稳定性。
