Yaffee S, Gold A, Sampugna J
J Nutr. 1980 Dec;110(12):2490-6. doi: 10.1093/jn/110.12.2490.
Experiments were carried out on male rat fed ad libitum or starved for a period of 7 days. Plasma levels of free fatty acids (FFA) and the quantity and composition of fatty acids in total lipids of heart ventricular tissue in vivo were analyzed by gas liquid chromatography (GLC). In addition, FFA extraction ratios and uptake rates were determined in isolated perfused hearts using the classical Langendorff technique. After 7 days of starvation, distribution and concentrations obtained for total lipid fatty acids from heart ventricles of starved animals were substantially different from those of controls. In particular, 20:5 and 22:6 carbon polyunsaturated fatty acids were significantly elevated. Also, total plasma FFA level was elevated and FFA extraction ratios were increased as a result of prolonged starvation. Accumulation of 20- and 22-carbon polyunsaturated fatty acids observed in ventricles of starved rats may have resulted from increased availability and extraction of FFA.
实验在随意进食或饥饿7天的雄性大鼠身上进行。通过气相色谱法（GLC）分析体内心室组织总脂质中游离脂肪酸（FFA）的血浆水平以及脂肪酸的数量和组成。此外，使用经典的Langendorff技术在离体灌注心脏中测定FFA提取率和摄取率。饥饿7天后，饥饿动物心室总脂质脂肪酸的分布和浓度与对照组有很大不同。特别是，碳链为20:5和22:6的多不饱和脂肪酸显著升高。此外，由于长期饥饿，血浆FFA总水平升高，FFA提取率增加。在饥饿大鼠心室中观察到的20碳和22碳多不饱和脂肪酸的积累可能是由于FFA的可用性和提取增加所致。
