Observations on the mechanism for the active extrusion of lithium in mammalian non-myelinated nerve fibres.

1. A study has been made of the O2 consumption, and the corresponding efflux of labelled phosphate, from the non-myelinated fibres of the desheathed rabbit vagus nerve at 37 degrees C in Locke solutions in which various ions were substituted for Na, and also in the presence of ouabain. 2. Switching from Na-Locke solution to Li-Locke solution produced a small transient decrease in the resting O2 consumption (of 14%), which rapidly recovered to its original value. This was accompanied by an initial brief rise followed by a maintained fall in the resting phosphate efflux. 3. In Li-Locke solution, ouabain (100 microM) produced a fall in the resting O2 consumption of 40%, i.e. similar to that produced in Na-Locke solution. Any depression of the resting phosphate efflux was absent or small. 4. In choline-Locke solution, in Tris-Locke solution, in K-Locke solution or in sucrose-Locke solution the resting O2 consumption, which fell by 30-40%, was insensitive to the addition of ouabain (100 microM). 5. Addition of either Na ions or of Li ions partially restored respiration in choline-Locke solution, Li being an order of magnitude less effective than Na. 6. In choline-Locke solution the internal K content was not affected by ouabain. However, if Li (77 mM) was present in the bathing solution ouabain (100 microM) produced a 30% fall in the internal K content. 7. It is concluded that these effects of Li, and their alteration by ouabain, reflect the activity of a mechanism for the active extrusion of Li ions. It is suggested that the mechanism for the active extrusion of Li is the same as that for Na. 8. There also seems to be a site for Li that controls the phosphate efflux and which is half-maximally activated with external Li concentrations of about 2-4 mM.