Burridge K, McCullough L
J Supramol Struct. 1980;13(1):53-65. doi: 10.1002/jss.400130106.
The role of alpha-actinin in the attachment of actin to plasma membranes has been investigated. Specific antibody staining of SDS gels has indicated that alpha-actinin is a major component in isolated plasma membranes prepared from three different cell types by two different procedures. Using specific extraction conditions, most of the alpha-actinin can be selectively extracted from the membranes with relatively little parallel release of actin. This selective dissociation of alpha-actinin from the plasma membrane leads us to conclude that alpha-actinin is present in these membrane preparations, because it is bound to actin, and that alpha-actinin does not form a direct link between actin and the membrane.
已经对α-辅肌动蛋白在肌动蛋白与质膜附着中的作用进行了研究。SDS凝胶的特异性抗体染色表明，α-辅肌动蛋白是通过两种不同方法从三种不同细胞类型制备的分离质膜中的主要成分。使用特定的提取条件，大部分α-辅肌动蛋白可以从膜中被选择性提取，而肌动蛋白的平行释放相对较少。α-辅肌动蛋白与质膜的这种选择性解离使我们得出结论，α-辅肌动蛋白存在于这些膜制剂中，因为它与肌动蛋白结合，并且α-辅肌动蛋白不会在肌动蛋白和膜之间形成直接联系。
