Rodighiero G, Marciani Magno S, Dell'Acqua F, Vedaldi D
Farmaco Sci. 1978 Sep;33(9):651-66.
The in vitro formation and properties of the molecular complex between mitomycin C and native DNA were examined by means of various experimental methods; the data obtained indicate that the complex is extremely weak and that the chromophoric moiety of the antibiotic is not involved in its formation. The alkylating activity of mitomycin C was also studied using 3H-mitomycin C; while monofunctional alkylation increases almost in parallel with the concentration of the antibiotic, the difunctional alkylation, causing inter-strand cross-linkages in DNA, rapidly reaches a maximum and then remains constant even when increasing the concentration of the antibiotic and monofunctional alkylation. On the basis of these results, the currently accepted molecular model of the mitomycin--DNA interaction must be revised; a new model of this interaction is presented, which is in better agreement with the properties of mitomycin C and with the latest findings on the subject.
通过各种实验方法研究了丝裂霉素C与天然DNA之间分子复合物的体外形成及其性质；所获得的数据表明该复合物极其微弱，且抗生素的发色部分不参与其形成。还使用³H-丝裂霉素C研究了丝裂霉素C的烷基化活性；虽然单功能烷基化几乎与抗生素浓度平行增加，但导致DNA链间交联的双功能烷基化迅速达到最大值，然后即使增加抗生素浓度和单功能烷基化，也保持恒定。基于这些结果，目前被接受的丝裂霉素与DNA相互作用的分子模型必须修订；提出了这种相互作用的新模型，它与丝裂霉素C的性质以及该主题的最新发现更一致。
