Valeev A K
Mol Biol (Mosk). 1980 Nov-Dec;14(6):1234-42.
Two alternative models of DNA molecular weight distribution due to induced single strand breaks are considered. Some analytical formulae for molecular weight distribution were deduced and on their basis the sedimentation curves were constructed. The first model declares the subunite structure of DNA. The breaks are induced by chemical or biochemical agents randomly and molecules degrade in points of the subunite connections as well. The other model declares the random of primary induced breaks and breaks of molecular fragments in half if they are longer than any steady size. These latter breaks can occur repeatedly if the sizes of the halves are more than the steady size. The constructed sedimentation curves of the two models were compared with experimental ones. It has been shown that the second model can explain qualitatively some patterns of the sedimentogrammes obtained with high speed centrifugation as the first one.
考虑了由于诱导单链断裂导致的DNA分子量分布的两种替代模型。推导了分子量分布的一些解析公式，并在此基础上构建了沉降曲线。第一个模型表明了DNA的亚基结构。断裂是由化学或生化试剂随机诱导的，分子也在亚基连接点处降解。另一个模型表明了初级诱导断裂的随机性，以及如果分子片段长于任何稳定大小，则将其断裂成两半。如果两半的大小超过稳定大小，这些后一种断裂可以重复发生。将这两种模型构建的沉降曲线与实验曲线进行了比较。结果表明，第二个模型在定性上可以像第一个模型一样解释高速离心得到的沉降图的一些模式。
