培养的共济失调毛细血管扩张症成纤维细胞样细胞中的非预定DNA合成。

Unscheduled DNA synthesis in cultured ataxia telangiectasia fibroblast-like cells.

作者信息

Vincent R A, Fink A J, Huang P C

出版信息

Mutat Res. 1980 Sep;72(2):245-9. doi: 10.1016/0027-5107(80)90038-x.

Abstract

The capacity of cultured fibroblast-like cells from 5 ataxia telangiectasia (AT) patients to repair gamma-ray-induced damage formed in DNA under aerobic conditions was measured by unscheduled DNA synthesis (UDS). The AT cultures exhibited generally reduced UDS compared to the normal cultures for gamma-ray doses of 4--12 kR. Reduced UDS in the AT cultures was observed for repair-incubation periods at which UDS was not saturated (0.5 and 1 h), and for periods characterized by maximal amounts of UDS in both the normal and AT cultures (2 and 4 h). The results suggest a class of lesions repairable in the normal cells by UDS within 1 h may persist in the AT cells for 4 h or longer.

摘要

通过非预定DNA合成（UDS）测定了5例共济失调毛细血管扩张症（AT）患者培养的成纤维细胞样细胞在有氧条件下修复γ射线诱导的DNA损伤的能力。对于4-12 kR的γ射线剂量，与正常培养物相比，AT培养物的UDS普遍降低。在UDS未饱和的修复孵育期（0.5和1小时）以及正常和AT培养物中UDS量最大的时期（2和4小时），均观察到AT培养物中UDS降低。结果表明，一类在正常细胞中1小时内可通过UDS修复的损伤可能在AT细胞中持续4小时或更长时间。