Rumjanek F D, Simpson A J
Mol Biochem Parasitol. 1980 Mar;1(1):31-44. doi: 10.1016/0166-6851(80)90039-0.
Live adult Schistosoma mansoni, maintained in vitro were able to absorb and utilize radiolabelled arachidonic acid, linolenic acid, 3-sn-phosphatidylcholine, tripalmitylglycerol and cholesterol in the culture medium. Differential centrifugation demonstrated that all these lipids were incorporated into the parasite's various membrane structures. Analysis by thin-layer chromatography of the labelled compounds which resulted from incubation with the labelled lipids revealed that arachidonic acid, linolenic acid and fatty acids of tripalmitylglycerol and 3-sn-phosphatidylcholine were present largely as triacylglycerol with smaller amounts of labelled diacylglycerol, phospholipids and fatty acids. The labelled polar head group of 3-sn-phosphatidylcholine was cleaved from the molecule during incorporation, which suggested that hydrolysis of complex lipids is an integral part of the absorption mechanism. Cholesterol was not apparently altered during incorporation or further metabolized. It was also observed that arachidonic acid was incorporated more readily than the other lipids, however, no prostaglandin biosynthesis was detected.
体外培养的成年曼氏血吸虫能够吸收并利用培养基中放射性标记的花生四烯酸、亚麻酸、3 - sn - 磷脂酰胆碱、三棕榈酸甘油酯和胆固醇。差速离心表明，所有这些脂质都被整合到寄生虫的各种膜结构中。对与标记脂质孵育后产生的标记化合物进行薄层层析分析发现，花生四烯酸、亚麻酸以及三棕榈酸甘油酯和3 - sn - 磷脂酰胆碱的脂肪酸主要以三酰甘油的形式存在，同时含有少量标记的二酰甘油、磷脂和脂肪酸。在整合过程中，3 - sn - 磷脂酰胆碱的标记极性头部基团从分子上被裂解下来，这表明复合脂质的水解是吸收机制的一个组成部分。胆固醇在整合过程中未明显改变，也未进一步代谢。还观察到花生四烯酸比其他脂质更容易被整合，然而，未检测到前列腺素的生物合成。
