Cordingley J S, Turner M J
Mol Biochem Parasitol. 1980 Apr;1(2):91-6. doi: 10.1016/0166-6851(80)90003-1.
In this paper we report the preliminary characterisation of some unusual small rRNAs isolated from Trypanosoma brucei. These molecules correspond closely in size and properties to the 5 S and 5.8 S rRNAs of other eukaryotes and in addition there are two further small RNAs which we designate 6.5 S RNA. These have been characterised by sucrose gradient centrifugation and electrophoresis in denaturing gels. The two 6.5 S RNAs may be distinguished from each other on the basis of their size and the different conditions required for their dissociation from the ribosomes. They are present in the same abundance as 5.8 S RNA suggesting that they occur once per ribosome.
在本文中，我们报告了从布氏锥虫中分离出的一些不寻常的小rRNA的初步特征。这些分子在大小和性质上与其他真核生物的5S和5.8S rRNA非常相似，此外还有另外两种小RNA，我们将其命名为6.5S RNA。通过蔗糖梯度离心和变性凝胶电泳对它们进行了表征。这两种6.5S RNA可以根据它们的大小以及从核糖体解离所需的不同条件加以区分。它们与5.8S RNA的丰度相同，这表明每个核糖体中各有一个。
