Malmquist E
Scand J Clin Lab Invest. 1980 May;40(3):233-7. doi: 10.3109/00365518009095572.
Rats were injected with paraquat (1,1'-dimethyl-4,4'-bipyridyliumdichloride, 35 mg/kg) and killed 14 or 48 h later. Twelve hours prior to sacrifice labelled choline or palmitic acid was injected. Immediately after sacrifice alveolar lavage was performed in situ and the lungs were removed and homogenized. Lipid extracts from lavage fluid and lung homogenates were prepared and their lecithin content analysed by thin layer chromatography. Lecithin was quantitated by phosphorous determination, the incorporation rate of the lecithin precursors in 'alveolar' and lung tissue lecithin was measured by liquid scintillation, and the fatty acid composition of lecithin was determined by gas liquid chromatography. In comparison with controls, the content of lecithin was reduced in lavage fluid from paraquat-injected rats. These animals also had reduced incorporation of choline in alveolar lecithin. The rate of incorporation of palmitic acid was the same in experimental animals and controls, so was the incorporation of choline in lung tissue lecithin. The findings suggest that paraquat interferes with the synthesis of alveolar lecithin by the phosphocholine transferase pathway, but not with the formation of lung tissue lecithin.
给大鼠注射百草枯（1,1'-二甲基-4,4'-联吡啶二氯化物，35毫克/千克），并在14或48小时后处死。在处死前12小时注射标记的胆碱或棕榈酸。处死之后立即原位进行肺泡灌洗，取出肺并匀浆。制备灌洗液和肺匀浆的脂质提取物，并通过薄层色谱法分析其卵磷脂含量。通过磷测定对卵磷脂进行定量，通过液体闪烁法测量“肺泡”和肺组织卵磷脂中卵磷脂前体的掺入率，并通过气相液相色谱法测定卵磷脂的脂肪酸组成。与对照组相比，注射百草枯的大鼠灌洗液中卵磷脂含量降低。这些动物肺泡卵磷脂中胆碱的掺入也减少。实验动物和对照组中棕榈酸的掺入率相同，肺组织卵磷脂中胆碱的掺入率也相同。这些发现表明，百草枯通过磷酸胆碱转移酶途径干扰肺泡卵磷脂的合成，但不干扰肺组织卵磷脂的形成。
