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豚鼠新皮层切片中的染料偶联及可能的电紧张偶联

Dye coupling and possible electrotonic coupling in the guinea pig neocortical slice.

作者信息

Gutnick M J, Prince D A

出版信息

Science. 1981 Jan 2;211(4477):67-70. doi: 10.1126/science.7444449.

DOI:10.1126/science.7444449
PMID:7444449
Abstract

Iontophoretic injection of the fluorescent dye Lucifer Yellow CH into single neurons of guinea pig neocortical slices resulted in the staining of more than one cell. Dye-coupled neuronal aggregates were found only in the superficial cortical layers and were often organized in vertical columns. Antidromic stimuli evoked all-or-none, subthreshold depolarizations in some superficial cells. These potentials were not eliminated by manganese and did not collide with spikes originating in the soma, suggesting that they arose from electrotonic interaction between superficial cortical neurons.

摘要

将荧光染料路西法黄CH通过离子电渗法注入豚鼠新皮质切片的单个神经元中,结果发现不止一个细胞被染色。染料偶联的神经元聚集体仅在皮质浅层被发现,且常常排列成垂直柱。逆向刺激在一些浅层细胞中诱发了全或无的阈下去极化。这些电位不受锰的影响,也不与源自胞体的动作电位发生碰撞,这表明它们是由皮质浅层神经元之间的电紧张相互作用产生的。

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