Schäfer R, Doehmer J, Rademacher I, Willecke K
Somatic Cell Genet. 1980 Nov;6(6):709-17. doi: 10.1007/BF01538970.
Chinese hamster and mouse cytoplasmic glutamate-oxaloacetate transaminase (GOT-1, EC 2.6.1.1) were separated by isoelectric focusing of cell extracts on thin-layer polyacrylamide plates. The expression of mouse GOT-1 was correlated with the retention of mouse chromosomes and the expression of 16 marker isozymes in 77 Chinese hamster x mouse somatic cell hybrids. Mouse GOT-1 expression was discordant to the expression of isozyme genes assigned to mouse chromosomes 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 16, 17, 18, and X. Furthermore it showed no concordant expression with mouse chromosomes 3, 13, and 15. The expression of mouse GOT-1 was highly concordant to mouse chromosome 19 in 21 hybrid clones analyzed karyotypically. Our data confirm by means of somatic cell genetics the chromosomal assignment of the mouse GOT-1 gene based on Mendelian genetics by Eicher, Reynolds, and Southard (3). The appearance of a heteropolymeric band in hybrid cells expressing mouse GOT-1 suggests that this enzyme molecule is probably composed of two subunits.
通过在薄层聚丙烯酰胺板上对细胞提取物进行等电聚焦，分离了中国仓鼠和小鼠的细胞质谷氨酸草酰乙酸转氨酶（GOT-1，EC 2.6.1.1）。在77个中国仓鼠×小鼠体细胞杂种中，小鼠GOT-1的表达与小鼠染色体的保留以及16种标记同工酶的表达相关。小鼠GOT-1的表达与分配到小鼠染色体1、2、4、5、6、7、8、9、10、11、12、14、16、17、18和X上的同工酶基因的表达不一致。此外，它与小鼠染色体3、13和15没有一致的表达。在通过核型分析的21个杂种克隆中，小鼠GOT-1的表达与小鼠染色体19高度一致。我们的数据通过体细胞遗传学证实了Eicher、Reynolds和Southard（3）基于孟德尔遗传学对小鼠GOT-1基因的染色体定位。在表达小鼠GOT-1的杂种细胞中出现杂聚带表明该酶分子可能由两个亚基组成。
