用于在胶片和细颗粒乳剂上进行3H-2-脱氧葡萄糖放射自显影的方法。

Methods for 3H-2-D-deoxyglucose autoradiography on film and fine-grain emulsions.

作者信息

Faraco-Cantin F, Courville J, Lund J P

出版信息

Stain Technol. 1980 Jul;55(4):247-52. doi: 10.3109/10520298009067248.

DOI:10.3109/10520298009067248

Abstract

Deoxyglucose labelled with 14C is utilized for the determination of regional glucose metabolism in the brain. In this paper, procedures are described in which 3H replaces the 14C marker for film autoradiography. A significant improvement of resolution is obtained as a result of the use of tritium and of various precautions to avoid diffusion of the soluble deoxyglucose. In addition, a method using a fine-grain emulsion (NTB-2) is presented. In this case, definition of labelled sites at a cytological level is obtained. Precise quantification is also possible through grain counting.

摘要

用¹⁴C标记的脱氧葡萄糖用于测定大脑中的区域葡萄糖代谢。本文描述了用³H替代¹⁴C标记物进行胶片放射自显影的方法。由于使用了氚并采取了各种防止可溶性脱氧葡萄糖扩散的预防措施，分辨率得到了显著提高。此外，还介绍了一种使用细颗粒乳剂（NTB - 2）的方法。在这种情况下，可以在细胞学水平上确定标记位点。通过颗粒计数也可以进行精确的定量分析。