Sholl S A, Wolf R C
Steroids. 1980 Aug;36(2):209-18. doi: 10.1016/0039-128x(80)90019-7.
For the purpose of describing the pathway by which estrones are synthesized in the rhesus monkey (Macaca mulatta) corpus luteum (CL), CL were obtained during the midluteal phase of the menstrual cycle and fragments incubated with equimolar amounts of [7-3H]pregnenolone plus [4-14C]progesterone. Metabolites including 3H-progesterone, 3H,14C-20 alpha-dihydroprogesterone, 3H,14C-17-hydroxyprogesterone, 3H-estrone and 3H-estradiol-17 beta appeared in the medium during the first 20 minutes of incubation. 3H,14C-Androstenedione was not consistently noted until after 60 minutes. Despite the fact that the 14C/3H-17-hydroxyprogesterone ratio quickly approached a constant value in the medium, 14C-estrogens were not detected in the medium or tissue fragments suggesting that progesterone was not a principal precursor for estrogen synthesis. As evidenced by the observation that the 14C/3H-progesterone ratio was significantly higher in luteal fragments than the 17-hydroxyprogesterone ratio, 17-hydroxyprogesterone appeared to be synthesized from pregnenolone both by way of progesterone and by another route which did not include progesterone. C21- and C18-Steroids were more concentrated in tissue fragments after 120 minutes of incubation than in the medium indicating that these steroids were sequestered by luteal tissue.
为了描述恒河猴（猕猴）黄体（CL）中雌酮的合成途径，在月经周期的黄体中期获取CL，并将其碎片与等摩尔量的[7-³H]孕烯醇酮加[4-¹⁴C]孕酮一起孵育。在孵育的前20分钟内，培养基中出现了包括³H-孕酮、³H,¹⁴C-20α-二氢孕酮、³H,¹⁴C-17-羟孕酮、³H-雌酮和³H-雌二醇-17β在内的代谢产物。直到60分钟后才持续检测到³H,¹⁴C-雄烯二酮。尽管培养基中¹⁴C/³H-17-羟孕酮的比值迅速接近恒定值，但在培养基或组织碎片中未检测到¹⁴C-雌激素，这表明孕酮不是雌激素合成的主要前体。黄体碎片中¹⁴C/³H-孕酮的比值明显高于17-羟孕酮的比值，这表明17-羟孕酮似乎是由孕烯醇酮通过孕酮途径和另一条不包括孕酮的途径合成的。孵育120分钟后，C21和C18类固醇在组织碎片中的浓度高于培养基，这表明这些类固醇被黄体组织所隔离。
