Podojil M, Lívanský K, Prokes B, Wurst M
Folia Microbiol (Praha). 1978;23(6):444-7. doi: 10.1007/BF02885573.
Fatty acids from Chlorella vulgaris, Scenedesmus obliquus var. acutus and from a mixed culture of the two strains, Melnik, were converted to methyl esters, separated by gas chromatography, and identified by means of standards. The spectrum of fatty acids included both saturated and unsaturated acids (with odd and even numbers of carbon atoms) from C12 to C22. Fatty acids C16:0, C18:0 and C20:3 were the major components in all cultures. Pure strains differed from the mixed culture in the production of C18:1, C12:0 and C19:2 acids; the first of these was present in higher amounts in pure cultures only, the latter two being found in the mixed culture. The level of lipids was lower as compared to the literature data and their extractability was affected by the manner of preparation of algae and extraction conditions.
将来自普通小球藻、斜生栅藻尖锐变种以及这两种菌株的混合培养物（梅尔尼克）的脂肪酸转化为甲酯，通过气相色谱法进行分离，并借助标准品进行鉴定。脂肪酸谱包括从C12到C22的饱和脂肪酸和不饱和脂肪酸（碳原子数为奇数和偶数）。脂肪酸C16:0、C18:0和C20:3是所有培养物中的主要成分。纯菌株在C18:1、C12:0和C19:2酸的产生方面与混合培养物不同；其中第一种仅在纯培养物中含量较高，后两种则在混合培养物中被发现。与文献数据相比，脂质水平较低，并且它们的可提取性受藻类制备方式和提取条件的影响。
