在恒定细胞密度装置中培养的斜生栅藻的脂质含量和脂肪酸组成

Lipid content and fatty acid composition of green algae Scenedesmus obliquus grown in a constant cell density apparatus.

作者信息

Choi K J, Nakhost Z, Barzana E, Karel M

机构信息

Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139, USA.

出版信息

Food Biotechnol. 1987;1(1):117-28. doi: 10.1080/08905438709549660.

DOI:10.1080/08905438709549660

PMID:11539709

Abstract

The lipids of alga Scenedesmus obliquus grown under controlled conditions were separated and fractionated by column and thin-layer chromatography, and fatty acid composition of each lipid component was studied by gas-liquid chromatography (GLC). Total lipids were 11.17%, and neutral lipid, glycolipid and phospholipid fractions were 7.24%, 2.45% and 1.48% on a dry weight basis, respectively. The major neutral lipids were diglycerides, triglycerides, free sterols, hydrocarbons and sterol esters. The glycolipids were: monogalactosyl diglyceride, digalactosyl diglyceride, esterified sterol glycoside, and sterol glycoside. The phospholipids included: phosphatidyl choline, phosphatidyl glycerol and phosphatidyl ethanolamine. Fourteen fatty acids were identified in the four lipid fractions by GLC. The main fatty acids were C18:2, C16:0, C18:3(alpha), C18:1, C16:3, C16:1, and C16:4. Total unsaturated fatty acid and essential fatty acid compositions of the total algal lipids were 80% and 38%, respectively.

摘要

在可控条件下培养的斜生栅藻脂质，通过柱色谱和薄层层析进行分离和分级，并采用气液色谱法（GLC）研究各脂质成分的脂肪酸组成。以干重计，总脂质含量为11.17%，中性脂质、糖脂和磷脂级分分别为7.24%、2.45%和1.48%。主要的中性脂质有甘油二酯、甘油三酯、游离甾醇、烃类和甾醇酯。糖脂包括：单半乳糖基甘油二酯、双半乳糖基甘油二酯、酯化甾醇糖苷和甾醇糖苷。磷脂包括：磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺。通过气液色谱法在四种脂质级分中鉴定出14种脂肪酸。主要脂肪酸为C18:2、C16:0、C18:3(α)、C18:1、C16:3、C16:1和C16:4。藻类总脂质的总不饱和脂肪酸和必需脂肪酸组成分别为80%和38%。