Pintér K, Jancsó A, Biró E N
Acta Biochim Biophys Acad Sci Hung. 1980;15(3):217-22.
A simplified procedure for the preparation of alpha-actinin from rabbit skeletal muscle is described. Use is made of the observation that actin is completely eliminated by denaturation, when myofibrils are dissolved in 0.6 M MgCl2 and thereafter MgCl2 is removed by dialysis.
本文描述了一种从兔骨骼肌中制备α-辅肌动蛋白的简化方法。利用了这样一个观察结果:当肌原纤维溶解于0.6M氯化镁中,随后通过透析去除氯化镁时,肌动蛋白会因变性而被完全去除。