Mano S
Acta Pathol Jpn. 1980 Nov;30(6):977-94. doi: 10.1111/j.1440-1827.1980.tb03285.x.
In vitro transformation of brain cells cultured from various age rats by human adenovirus type 12 (Ad-12) was studied. In cultured brain cells, five cell types could be identified light microscopically. In astrocytes, Ac-2 and Ac-1, astroprotein was demonstrated by immunofluorescence technique. In the former, intracytoplasmic microfilaments were recognized by electron microscopy. Undifferentiated cells (Ud), ependymoglioblasts (EGb) and Ac-1, grew prominently in cultures from young animals or of early passage levels. Ac-2 and fibroblasts were prominent in cultures from aged animals or of late passage levels. Transformed foci appeared within about 20 days after inoculation of Ad-12 and often located in close association with the sheets of Ac-1. Transformed cells morphologically resembled EGb or transitional cells from EGb to Ac-1. Transformation rate was high in cultures from young animals or of early passage levels. Subcutaneous transplantation of these cells into newborn rats procued tumors whose histology was similar to Ad-12-induced rat brain tumor. T-Ag were detected both in transformed and transplanted tumor cells. It was concluded, that the target cells of Ad-12 in vitro correspond with immature brain cells (EGb and/or transitional cells from EGb to Ac-1 cells.
研究了人12型腺病毒（Ad-12）对不同年龄大鼠培养的脑细胞的体外转化作用。在培养的脑细胞中，通过光学显微镜可识别出五种细胞类型。在星形胶质细胞中，Ac-2和Ac-1，通过免疫荧光技术证实存在星形蛋白。在前者中，通过电子显微镜可识别出胞质内微丝。未分化细胞（Ud）、室管膜胶质母细胞（EGb）和Ac-1，在幼龄动物或传代早期的培养物中生长显著。Ac-2和成纤维细胞在老龄动物或传代后期的培养物中占优势。接种Ad-12后约20天内出现转化灶，且常与Ac-1片层紧密相连。转化细胞在形态上类似于EGb或从EGb到Ac-1的过渡细胞。幼龄动物或传代早期培养物中的转化率较高。将这些细胞皮下移植到新生大鼠体内可产生组织学类似于Ad-12诱导的大鼠脑肿瘤的肿瘤。在转化细胞和移植肿瘤细胞中均检测到T抗原。得出的结论是，Ad-12在体外的靶细胞与未成熟脑细胞（EGb和/或从EGb到Ac-1细胞的过渡细胞）相对应。
